Toyo Kohan’s DNA chips are fixed on a silicon substrate coated by DLC (Diamond Like Carbon) and chemically processed by surface treating technology.
We are actively promoting the development of testing kits based on the results of state-of-the-art genome research, mainly in the medical field, so that they can be deployed for medical treatment as quickly as possible, thereby contributing to the provision of more effective treatments and longer healthy life spans.
The system can also be applied to testing and research across a wide range of fields, including healthcare and food.
Since the DNA and protein are firmly bound to the substrate via covalent bond, it is possible to obtain highly reliable analysis results in tests and research in a myriad of fields including medicine, healthcare, agriculture, stock raising, and fishing.
What is GeneSilicon?
GeneSilicon® is a DNA chip that brings together our surface treatment technology. With a single chip, multiple genes can be tested simultaneously and highly reliable analysis results can be obtained.
(gene analysis kit)
Features
1. Simple operation
Preparing reagents is extremely easy, because multiple genes are tested at the same time.
2. Highly accurate
High discrimination accuracy is achieved by capturing the target DNA region in two steps: PCR and hybridization.
3. Low risk
A single DNA chip can discern multiple genes, facilitating management by linking specimen ID to the DNA chip.
4. Popularization of testing
Suitable for wide use, the kit format contributes to the broad adoption of various kinds of testing.
Compliant with The Law on Securing Quality, Efficacy and Safety of Products Including Pharmaceuticals and Medical devices
- Registered in vitro diagnostics manufacturer (Registration number: 35AZ200013)
- Licensed marketing approval of in vitro diagnostics (License number: 13E1X00004)
What GeneSilicon can do
SNPs gene test
- Performs PCR amplification of the DNA region to be tested using fluorescent-labeled primers.
- Hybridizes the probe DNA on GeneSilicon® with the PCR product.
- The signal is detected by a dedicated device to determine whether wild type or mutant type.
Mutant gene test
- PCR amplification is performed using DNA extracted from tumor cells, formalin-fixed paraffin-embedded sections (FFPE), etc.
- Inhibits nonspecific hybridization of blocker DNA to PCR products.
- GeneSilicon® specifically detects and determines mutant DNA.
Chimera gene test
- Single-strand PCR amplification of the DNA regions of fusion sites and mutation sites using fluorescent-labeled primers.
- The PCR product is hybridized with the probe DNA of the chimeric, wild-type, and mutant genes on GeneSilicon®.
- Presence or absence of chimeric genes and mutations is simultaneously detected and determined.
Microsatellite gene test
- Performs PCR amplification of the DNA region to be tested using fluorescent-labeled primers.
- The PCR product is hybridized with probe DNA of microsatellite genes with different numbers of bases on GeneSilicon®.
- The signal of the entire probe DNA is detected, and the microsatellite is determined by comparing the tumor specimen with a normal area using dedicated application software.
DNA methylation genetic test
- After bisulfite treatment, PCR amplification of the DNA region to be tested is performed using fluorescent-labeled primers.
- The presence of methylation is detected and determined by hybridizing the probe DNA on GeneSilicon® with the PCR product.
List of products
(Research Use Only Reagent)
| Product Number | Product Name | Packaging |
|---|---|---|
| ID00017 | GeneSiliconⓇ DNA Chip Kit UGT1A1 Kit A | 96 tests |
| ID00018 | GeneSiliconⓇ DNA Chip Kit UGT1A1 Kit B | 1 box |
| CD00045 | GeneSiliconⓇ DNA Chip Kit MPN Ver.2 Kit A | 96 tests |
| CD00046 | GeneSiliconⓇ DNA Chip Kit MPN Ver.2 Kit B | 1 box |
| RD00050 | GeneSiliconⓇ DNA Chip Kit SF3B1 (Research Use Only Reagent) Kit A | 96 tests |
| RD00049 | GeneSiliconⓇ DNA Chip Kit SF3B1 (Research Use Only Reagent) Kit B | 1 box |
| RD00051 | GeneSiliconⓇ DNA Chip Kit MYD88/CD79B (Research Use Only Reagent) Kit A | 96 tests |
| RD00052 | GeneSiliconⓇ DNA Chip Kit MYD88/CD79B (Research Use Only Reagent) Kit B | 1 box |
Equipment for exclusive use with GeneSilicon
Automated equipment for exclusive use with GeneSilicon
Batch processing of multiple chips
Tests 32 DNA chips in a single process.
Rapid detection
From equipment set-up to completion of detection takes approximately 40 to 80 minutes.
Parameters for processes such as hybridization, washing, and detection can be set as desired.
Bar-code reader for reliable identification of specimens
QR code reading function inside the instrument ensures reliable matching with detection results.
Compact integrated system (W820 × H434 × D517 mm)
Hybridization, washing, and detection integrated into one compact unit.
The compact size of this desktop device allows it to be installed in any location using regular power supply (100V AC).
Registration No.: (13B3X10232HT3201)
Research Use Only products: HySHOT HT-32